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Quick Start Bradford Protein Assay Instruction Manual For technical service call your local Bio-Rad office, or in the US, 1-800-4BIORAD (1-800-424-6723) Table of Contents Section 1 Introduction 1 1.1 Principle 1 1.2 Selecting a Protein Standard 5 1.3 Product

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Bradford protein assay

The Bradford protein assay was developed by Marion M. Bradford in 1976.[1] It is a quick and accurate[2] spectroscopic analytical procedure used to measure the concentration of protein in a solution. The reaction is dependent on the amino acid composition of the measured proteins.

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Figure 2. Typical computer and plate reader set-up for the Bradford total protein and ELISA immunoreactive protein assays. Software that either comes with the plate reader or may be purchased separately may be used to assist in the analysis of the data. We use

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BIO-RAD BRADFORD TOTAL PROTEIN ASSAY (Bradford, 1976 & Wright et al., 1996) Introduction This procedure is used to measure protein concentration in samples that were extracted for glomalin, using either the total or easily extractable extraction


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Why use BSA as standard in bradford assay?

BSA is often used for the reasons already given, but it is not always the best choice for your particular protein. Each protein responds a little differently in the Bradford assay ...

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Protein determination by the Bradford method

The Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly accurate and samples that are out of range can be retested within minutes. The Bradford is recommended for general use, especially for determining protein content of cell fractions and assesing protein concentrations for gel electrophoresis.