· Yang L, Jiang Y, Shi L, Zhong D, Li Y, Li J, Jin R Curr Protein Pept Sci, 21(1):66-77, 01 Jan 2020 Cited by: 1 article | PMID: 31424367
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· Specificity of NGS on spiked samples In order to develop a reliable method, we selected four SNPs in the β-globin locus, that are common in the Cypriot population, rs3834466_IIɛ, rs968857_3 ...
· PCR was performed using diluted cDNA products. The reactions were incubated in a Biometra TProfessional Thermocycler for 5 min at 95 C, followed by 40 cycles of 15 sec at 94 C, 30 sec at 60 C and 30 sec at 72 C. All reactions were run in duplicate. The
· Modern Food Science and Technology 2017, Vol.33, No.3 O157 ,,,, （,, ...
Yan HC, Daise M, Sun J, DeLisser HM. IFN-γ and TNF-α induce redistribution of PECAM-1 (CD31) on human endothelial cells. J Immunol 1995; 154: ...
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Environmentally relevant mixture of S-metolachlor and its two metabolites affects thyroid metabolism in zebrafish embryos …
· Our interest was mainly focused on thyroid disruption, based on findings of Jin et al. (2011), and Yang et al. (2016) who linked this type of disruption with some chloroacetanilide pesticides. In the current work, an integrative multi-endpoint approach was used to address the complexity of the thyroid system and its importance, especially during the early development of zebrafish.
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· Liu YF, Li MY, Yan YP, Wei W, Li B, Pan HY, Yang ZM and Liang XH: ERα- dependent stimulation of Lcn2 in uterine epithelium during mouse early pregnancy. Reproduction. 159:493–501. 2020. View Article: Google Scholar: PubMed/NCBI 29
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Compared to other mammalian species, porcine oocytes and embryos are characterized by large amounts of lipids stored mainly in the form of droplets in the cytoplasm. The amount and the morphology of lipid droplets (LD) change throughout the preimplantation development, however, relatively little is known about expression of genes involved in lipid metabolism of early embryos. We compared ...
· Getah virus (GETV), a mosquito-borne virus that mainly infects horses and pigs, has emerged and spread in China. We developed a highly specific and reproducible TaqMan probe-based quantitative reverse transcription PCR (RT-qPCR) assay targeting the non-structural protein 1 of GETV, whose detection limit is 25.5 copies/µL, which is 100-fold higher than that of conventional RT …
· Thermal cycling was performed with a TProfessional Thermocycler (Biometra, Göttingen, Germany) under the following conditions: 94 C for 5 min, 35 cycles at 94 C for 45 sec, annealing at 50–55 C depending on the marker for 30 sec ...
· Microsatellites were PCR amplified in a Biometra TProfessional Thermocycler (Biometra Corp., Germany) using the following conditions: the reaction mixture was 20 μL, containing 2.0 μL of 10 × reaction buffer (100 mM Tris–HCl, pH 8.3), 1.6 μL 2.5 mmol L −1 L
Zhiqiang Yan, 1,2,3 Fang Yang, 4 Zhu Hong, 4 Shishun Wang, 5 Zhang Jinjuan, 6 Bing Han, 1,3 Rujia Xie, 1,3 Feiyun Leng, 7 and Qin Yang 1,3 1 Department of Pathophysiology, Basic Medical College, Guizhou Medical University, Guiyang, Guizhou 550004, China
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· All reactions were performed using a TProfessional Standard Gradient Thermocycler (Biometra , Goettingen, Germany). The following thermal cycling protocol was used: an initial melting at 94°C for 5 min followed by 35 cycles of 94°C for 50 s, 45 s at the optimum annealing temperature , and 72°C for 50 s.
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PCR was done in a thermocycler Biometra TProfessional (Biometra, Göttingen, Germany) under the following conditions: initial denaturing for 30 s at 98 C, 25 cycles of denaturing for 10 s at 98 C, annealing for 30 s at 60 C, and elongation for 30 s at 72 C, final
· Table S1: List of Chrysanthemum × morifolium cultivars used in the present study, their molecular identities and phenotyping data. * represents the 80 cultivars used for validation of the establishment method of molecular identities for other 400 cultivars.Table S4: The best –lg-transformed P-values and marker names for significant associations found in the present study. ...
Zhonghua Yan Ke Za Zhi. 32:405–416. 1996 Chinese. PubMed/NCBI 10 Xu X, Li B, Wang Y, Gao F and Zhang Z: Retinoblastoma spontaneous regression: Clinical and histopathologic analysis. Zhonghua Yan Ke Za Zhi. 50:729–732. 2014 Chinese. 11
· Forty cases of confirmed avian influenza virus subtype H7N9 infection were treated at the First Affiliated Hospital of Zhejiang University (China) during March–April 2013. Most of the patients were critically ill and required admission to an intensive care unit , wheretreatment can be divided into antiviral treatment, microbiota-targeted therapies, and immunotherapy.
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· PCR was performed in a TProfessional Trio PCR System (Biometra, Göttingen, Germany). The reaction procedure included denaturation at 95 °C for 5 min, followed by 34 cycles of denaturation at 94 °C for 1 min, anneal at 55 °C for 1 min and extension …
· We are grateful to Mr. Shou-yang Liu and Mr. Yi-ling Zhu, Mr. Ke-jian Yan for their help with the collection and identification of the Uncaria specimens. We appreciate the essential comments provided by Dr. Hui Yao and all others that much improved the
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· Qiang YZ, Tong Q, Wang F, Wu PC, Yang SL, Gong Y, et al. Use of a rapid mismatch PCR method to detect gyrA and parC mutations in ciprofloxacin resistant clinical of Escherichia coli. The Journal of Antimicrobial Chemotherapy. 2002; 49:549–52.  
Yao YZ, Yang J, Wang DW, Zhou F, Cai XT, Lu WG, et al. The aqueous extract of Lycopus Lucidus Turcz ameliorates streptozotocin-induced diabetic renal damage via inhibiting TGF-β1 signaling pathway. Phytomedicine. 2013; 20:1160–7. 
· Yan LX, Huang XF, Shao Q, Huang MY, Deng L, Wu QL, et al. MicroRNA miR-21 overexpression in human breast cancer is associated with advanced clinical stage, lymph node metastasis and patient poor prognosis. RNA. 2008; 14 (11):2348–60. doi: 10.1261 
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